EDVOTEK Using CRISPR to Treat Cystic Fibrosis
EDVOTEK Using CRISPR to Treat Cystic Fibrosis
Product code: PP00052457
Product Description
In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.
- Group Size: For 8 Lab Groups
- Storage: Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended
- Time Required: Complete in 45 minutes
The kit includes:
- Instructions,
- Ready-to-Load QuickStrip™ DNA Samples
- UltraSpec-Agarose™
- Electrophoresis Buffer (50X)
- 10X Gel Loading Solution,
- FlashBlue™ DNA Stain
Additional required equipment & materials (not supplied with the kit):
- DNA Electrophoresis tank and accessories including casting trays and special power supply
- Micropipettes: 5-50 µl (Optional)
- Balance
- White Light Box
- Microwave or Hot Plate
*PLEASE NOTE: This kit has been developed for being suitable for use in education only and is not for diagnostic purposes*
- An excellent activity to demonstrate genetics and Cystic Fibrosis Diagnostics and Treatment
- A-Level Genetics: Principles & Applications of Electrophoresis
- When visualising completed gels using a white light box, cover the light box with clingfilm to prevent any damage.
Further Information
- Age Recommended from
- 14 Years
- Age Suitable from
- 14 Years
- Brand
- Edvotek
- Experiment Title
- Using CRISPR to Treat Cystic Fibrosis
- Number of Lab Groups per Kit
- 8 Lab Groups
- Product Type
- Electrophoresis Kit
- Storage
- Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended.
- Time Required
- Complete in 45 minutes
Documents
Useful Links
In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.
- Group Size: For 8 Lab Groups
- Storage: Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended
- Time Required: Complete in 45 minutes
The kit includes:
- Instructions,
- Ready-to-Load QuickStrip™ DNA Samples
- UltraSpec-Agarose™
- Electrophoresis Buffer (50X)
- 10X Gel Loading Solution,
- FlashBlue™ DNA Stain
Additional required equipment & materials (not supplied with the kit):
- DNA Electrophoresis tank and accessories including casting trays and special power supply
- Micropipettes: 5-50 µl (Optional)
- Balance
- White Light Box
- Microwave or Hot Plate
*PLEASE NOTE: This kit has been developed for being suitable for use in education only and is not for diagnostic purposes*
- An excellent activity to demonstrate genetics and Cystic Fibrosis Diagnostics and Treatment
- A-Level Genetics: Principles & Applications of Electrophoresis
- When visualising completed gels using a white light box, cover the light box with clingfilm to prevent any damage.
Further Information
- Age Recommended from
- 14 Years
- Age Suitable from
- 14 Years
- Brand
- Edvotek
- Experiment Title
- Using CRISPR to Treat Cystic Fibrosis
- Number of Lab Groups per Kit
- 8 Lab Groups
- Product Type
- Electrophoresis Kit
- Storage
- Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the refrigerator is recommended.
- Time Required
- Complete in 45 minutes
